ABSTRACT
Objective To observe the effects of curcumin (Cur) on vasculogenic mimicry (VM) and PI3K pathway in choroid melanoma cell line OCM-1 in vitro 3-dimentional culture.Methods Three-dimensional culture of OCM-1 cells was performed for the observation of VM formation and the influence of concentration gradient (0μmol · L-1,10 μmol · L-1,20 μmol · L-1,30 μmol · L-1,40 μmol · L-1) Cur on VM,and immunohis to chemistry staining was used to detect the expression of PI3K and EphA2 in OCM-1 cell line.Results Cur could markedly inhibit the formation of VM in choroid melanoma cell line OCM-1 in vitro 3-dimensional culture with a dose-dependent manner.Cur suppressed EphA2 and PI3K expression in a concentration-dependent manner,and the difference between groups with 0 μmol · L-1 Cur group was significant statistically (all P =0.000),but there was no significant difference between 30 μmol · L-1and40 μmol · L-1 group (EphA2,P=0.300;PI3K,P=0.188).Conclusion Cur can significantly inhibit VM formation in choroid melanoma cell line OCM-1,of which the mechanism may be related to the downregulation expression of EphA2 and PI3K,thus inhibiting PI3K signaling pathway.
ABSTRACT
<p><b>AIM</b>To study the effect of bupleurum root on epileptic seizure.</p><p><b>METHODS</b>The rabbits and rats were injected by pilocarpine as epileptic models, and observed the effect of bupleurum on the electroencephalogram (EEG) and hippocampal slice by electroencephalograph and glass microelectrode extracellularly.</p><p><b>RESULTS</b>The seizure time and duration of each major seizure of epilepsy were significantly shortened and the interval of seizure significantly prolonged (P < 0.05) after intraabdominal injection of bupleurum root. After instilling the injection of bupleurum root onto the slices could reduce the amplitude of evoked field potential in epileptic hippocampal slices remarkably, the average of fall is 20.41%, and restore in 6.86 minutes on average (P < 0.001).</p><p><b>CONCLUSION</b>Bupleurum root can inhibit the brain electrical activities in epileptic model, it is suggest that bupleurum has the distinct effect of antiepilepsy.</p>